Biomarker Quantitation

At Swiss BioQuant, we have extensive experience in analyzing biomarkers from various sample matrices, including serum, plasma, cerebrospinal fluid (CSF), urine, cell culture, tissue extracts, and bronchoalveolar lavage fluid (BALF). Determining biomarkers can be valuable for:

• Identifying responders versus non-responders
• Expanding indications for approved drugs or drug repurposing
• Predicting drug toxicity
• Therapeutic drug monitoring
• Investigating pharmacodynamics
• CoU for biomarker assay development and following FDA guidance on biomarker quantitation.
  

HPLC-MS/MS Biomarker Assays

 We develop and validate highly selective bioanalytical methods for the simultaneous quantitation of multiple biomarker peptides or epitope peptides of monoclonal antibodies in serum or plasma. Using 2D-UPLC-MS/MS, we achieve the high selectivity, accuracy, and precision required for biomarker quantitation.

LBA Biomarker assays

At Swiss BioQuant, we leverage the MSD (Meso Scale Discovery) platform to enhance our biomarker analysis capabilities. This advanced technology allows us to measure hundreds of existing panels and multiplex up to 10 analytes using a single sample as small as 30 µL. This high level of efficiency and precision ensures that we can provide comprehensive and accurate biomarker data even with rare matrices.

Our LBA (Ligand Binding Assay) biomarker services include:

• Development and Validation: We develop and validate highly selective bioanalytical methods tailored to your specific needs.
• High Throughput Screening: Our platform supports high throughput screening, enabling us to process large numbers of samples quickly and accurately.
• Custom Assay Development: We offer custom assay development to meet unique research requirements, ensuring that you get the most relevant and precise data.

Real Time PCR (qPCR)

We also utilize real-time PCR (Polymerase Chain Reaction) for biomarker investigation. This advanced technique allows us to detect and quantify specific RNA molecules in real-time, providing valuable insights into gene expression and molecular pathways. By leveraging real-time PCR, we can accurately identify and measure biomarkers, enhancing our ability to monitor disease progression and treatment efficacy.