Swiss BioQuant

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Protein and Peptide Analysis by HPLC-MS

The challenges for quantify proteins vs small molecules with HPLC-MS based assays are well recognized.

Some factors favoring some level of adoption is a rapid development timescale relative to ELISA tests, as well as the higher degree of specificity and ability to eliminate interferences.

Quantitation of antibodies or therapeutic proteins.

Universal Peptide: LLOQ is 100 ng/mL protein in animal serum w/o immunoprecipitation using 10 µL serum and 10 ng/mL w/ immunoprecipitation.